Algorithm based automatic contiguation of assembled sequences
abacas -r ref -q qs -p prog [OPTIONS]
abacas -r ref -q psf -e
reference sequence in a single fasta file
contigs in multi-fasta format
MUMmer program to use: 'nucmer' or 'promer'
pseudomolecule/ordered sequence file in fasta format
use default nucmer/promer parameters
int minimum length of exact matching word (nucmer default = 12, promer default = 4)
print ordered contigs to file in multifasta format
print contigs in bin to file
print a pseudomolecule without "N"s
int mimimum percent identity [default 40]
int mimimum contig coverage [default 40]
int minimum contig coverage difference [default 1]
int minimum contig length [default 1]
run tblastx on contigs that are not mapped
string (file name) print uncovered regions (gaps) on reference to file name
append contigs in bin to the pseudomolecule
prefix output files will have this prefix
pick primer sets to close gaps
int number of flanking bases on either side of a gap for primer design (default 350)
int Run mummer [default 1, use -R 0 to avoid running mummer]
Escape contig ordering i.e. go to primer design
Reference sequence is circular
ABACAS is intended to rapidly contiguate (align, order, orientate), visualize and design primers to close gaps on shotgun assembled contigs based on a reference sequence.
ABACAS uses MUMmer to find alignment positions and identify syntenies of assembled contigs against the reference. The output is then processed to generate a pseudomolecule taking overlapping contigs and gaps in to account. ABACAS generates a comparision file that can be used to visualize ordered and oriented contigs in ACT. Synteny is represented by red bars where colour intensity decreases with lower values of percent identity between comparable blocks. Information on contigs such as the orientation, percent identity, coverage and overlap with other contigs can also be visualized by loading the outputted feature file on ACT.
ABACAS IS Copyright (C) 2008-10 The Wellcome Trust Sanger Institute, Cambridge, UK.
This manual page was written by Andreas Tille <[email protected]>, for the Debian project (and may be used by others).