The genome and est sequence assembly system
The mira genome fragment assembler is a specialised assembler for sequencing projects classified as 'hard' due to high number of similar repeats. For expressed sequence tags (ESTs) transcripts, miraEST is specialised on reconstructing pristine mRNA transcripts while detecting and classifying single nucleotide polymorphisms (SNP) occuring in different variations thereof.
The assembler is routinely used for such various tasks as mutation detection in different cell types, similarity analysis of transcripts between organisms, and pristine assembly of sequences from various sources for oligo design in clinical microarray experiments.
The purpose of the mira executable is to assemble sequencing data.
mira [OPTION] manifest_file [manifest_file] ...
-c / --cwd=directory
Change working directory
-r / --resume
Resume an interrupted assembly
-h / --help
Print short help and exit
-v / --version
Print version and exit
A more extensive documentation is provided in the mira-doc package and can be found at /usr/share/doc/mira-assembler/DefinitiveGuideToMIRA.html.
You can also subscribe one of the MIRA mailing lists at
http://www.chevreux.org/mira_mailinglists.html
After subscribing, mail general questions to the MIRA talk mailing list:
To report bugs or ask for features, please use the new ticketing system at:
http://sourceforge.net/apps/trac/mira-assembler/
The author of the mira code is Bastien Chevreux <[email protected]>
This manual page was written by Andreas Tille <[email protected]> but can be freely used for any other distribution.