Detect trna genes in nucleotide sequences
aragorn [OPTION]... FILE
Search for tmRNA genes.
Search for tRNA genes. By default, all are detected. If one of -m or -t is specified, then the other is not detected unless specified as well.
Search for Metazoan mitochondrial tRNA genes. tRNA genes with introns not detected. -i, -sr switchs ignored. Composite Metazoan mitochondrial genetic code used.
Search for Mammalian mitochondrial tRNA genes. -i, -sr switchs ignored. -tv switch set. Mammalian mitochondrial genetic code used.
Same as -mt but low scoring tRNA genes are not reported.
Overlapping metazoan mitochondrial tRNA genes on opposite strands are reported.
Use the GenBank transl_table = [num] genetic code. Individual modifications can be appended using ,BBB=<aa> B = A,C,G, or T. <aa> is the three letter code for an amino-acid. More than one modification can be specified. eg -gcvert,aga=Trp,agg=Trp uses the Vertebrate Mitochondrial code and the codons AGA and AGG changed to Tryptophan.
Use standard genetic code.
Use composite Metazoan mitochondrial genetic code.
Use Vertebrate mitochondrial genetic code.
Use Invertebrate mitochondrial genetic code.
Use Yeast mitochondrial genetic code.
Use Mold/Protozoan/Coelenterate mitochondrial genetic code.
Use Ciliate genetic code.
Use Echinoderm/Flatworm mitochondrial genetic code
Use Euplotid genetic code.
Use Bacterial/Plant Chloroplast genetic code.
Use alternative Yeast genetic code.
Use Ascidian Mitochondrial genetic code.
Use alternative Flatworm Mitochondrial genetic code.
Use Blepharisma genetic code.
Use Chlorophycean Mitochondrial genetic code.
Use Trematode Mitochondrial genetic code.
Use Scenedesmus obliquus Mitochondrial genetic code.
Use Thraustochytrium Mitochondrial genetic code.
Do not search for mitochondrial TV replacement loop tRNA genes. Only relevant if -mt used.
Search for tRNA genes with 7 base C-loops only.
Search for tRNA genes with introns in anticodon loop with maximum length 3000 bases. Minimum intron length is 0 bases. Ignored if -m is specified.
Search for tRNA genes with introns in anticodon loop with maximum length [max] bases. Minimum intron length is 0 bases. Ignored if -m is specified.
Search for tRNA genes with introns in anticodon loop with maximum length [max] bases, and minimum length [min] bases. Ignored if -m is specified.
Same as -i, but allow tRNA genes with long introns to overlap shorter tRNA genes.
Same as -i, but fix intron between positions 37 and 38 on C-loop (one base after anticodon).
Same as -if and -io combined.
Same as -i, but report tRNA genes with minimum length [min] bases rather than search for tRNA genes with minimum length [min] bases. With this switch, [min] acts as an output filter, minimum intron length for searching is still 0 bases.
Assume that each sequence has a circular topology. Search wraps around each end. Default setting.
Assume that each sequence has a linear topology. Search does not wrap.
Double. Search both strands of each sequence. Default setting.
-s or -s+
Single. Do not search the complementary (antisense) strand of each sequence.
-sc or -s-
Single complementary. Do not search the sense strand of each sequence.
Lower scoring thresholds to 95% of default levels.
Change scoring thresholds to [num] percent of default levels.
Flag possible pseudogenes (score < 100 or tRNA anticodon loop <> 7 bases long). Note that genes with score < 100 will not be detected or flagged if scoring thresholds are not also changed to below 100% (see -ps switch).
Print out primary sequence.
Show secondary structure of tRNA gene primary sequence using round brackets.
Print out primary sequence in fasta format.
Print out primary sequence in fasta format only (no secondary structure).
Same as -fo, with sequence and gene numbering in header.
Same as -fo, with no spaces in header.
Same as -fo, with sequence and gene numbering, but no spaces.
Print out in Batch mode.
Use the stricter canonical 1-2 bp spacer1 and 1 bp spacer2. Ignored if -mt set. Default is to allow 3 bp spacer1 and 0-2 bp spacer2, which may degrade selectivity.
Verbose. Prints out information during search to STDERR.
Print out tRNA domain for tmRNA genes.
Restrict tRNA astem length to a maximum of 7 bases
Display message if predicted iso-acceptor species does not match species in sequence name (if present).
Display 4-base sequence on 3' end of astem regardless of predicted amino-acyl acceptor length.
Allow some divergence of 3' amino-acyl acceptor sequence from NCCA.
Allow some divergence of 3' amino-acyl acceptor sequence from NCCA, and display 4 bases.
Do not print configuration line (which switchs and files were used).
Repeat sequence name before summary information.
Print output to . If ['outfile] already exists, it is overwritten. By default all output goes to stdout.
aragorn detects tRNA, mtRNA, and tmRNA genes. A minimum requirement is at least a 32 bit compiler architecture (variable types int and unsigned int are at least 4 bytes long).
[FILE] is assumed to contain one or more sequences in FASTA format. Results of the search are printed to STDOUT. All switches are optional and case-insensitive. Unless -i is specified, tRNA genes containing introns are not detected.
Laslett, D. and Canback, B. (2004) ARAGORN, a program for the detection of transfer RNA and transfer-messenger RNA genes in nucleotide sequences Nucleic Acids Research, 32;11-16
Laslett, D. and Canback, B. (2008) ARWEN: a program to detect tRNA genes in metazoan mitochondrial nucleotide sequences Bioinformatics, 24(2); 172-175.